Project
No.: lzp-2018/1-0395
Period:
31 August 2018 – 31 August 2021
Project
costs: 300 000 EUR
Principal
investigator: Dr.
hist. Guntis Gerhards (University of Latvia)
Collaboration: Dr.
biol. Renāte
Ranka (Latvian Biomedical Research and Study Centre)
The aim of the project is to study burial customs in Iron Age
populations from Latvia (7th – 12th centuries AD) in the
light of gender, sex and status, employing archaeological materials and
bioarchaeological methods, as well as dietary (carbon and nitrogen)
isotope, and ancient DNA analyses. To achieve the aim,
archaeological cemetery populations with reasonably good preservation
of skeletal remains from different regions of Latvia will be analysed.
An innovative approach will be employed during the project by using
biomolecular (ancient DNA) and biochemical (isotope) methods for the
study of burial customs. The ancient DNA data will be used to determine
biological sex and kinship of the individuals, while isotope analysis
will reveal information about differences in diet and thus, access to
resources between different population, sex and age groups. The project
will generate considerable amount of unique data, as well as reconsider
the existing preconceptions about gender, sex and status in Middle and
Late Iron Age populations in Latvia. It is the first interdisciplinary
project of this scale in the archaeology of Latvia. The results of the
research will be presented in international conferences and published
in peer-reviewed media, including in Open Access. Using internationally
approved research methods and state of the art technologies will
further the development of archaeological research in Latvia. The
results of the project will be of importance not only to
archaeologists, but also researchers in other fields both in Latvia and
abroad, promoting the understanding of similarities and differences in
the history of Baltic and European countries.
The Project is in line with one of the
approved Latvia’s research priorities for the years
2018-2021, “Latvia's statehood, language and values, culture
and art”, which encourages the use of new methods and
technologies in studying archaeological heritage of Latvia. The
European Convention on the Protection of the Archaeological Heritage
(1992), which is also ratified by the Republic of Latvia (2003), states
that archaeological heritage is the source of common European memory,
as well as a valuable part of cultural heritage, the study of which
helps to understand the history of humanity.
Information
published 31.08.2018
Project activities
by period:
August
31, 2018 – December 31, 2018
- Data review was started on the middle and late
Latvian Iron Age
burial grounds (7th – 12th centuries AD), including
archaeological
reports, published data, etc., at the Repository of Archaeological
Material, Institute of Latvian History, University of Latvia (LULVI)
and the repository of archaeological material, Museum of National
History. Inclusion criteria for the burial grounds to be studied in
this project, were also set.
- Protocols were set for determination of age and sex
and other
parameters, as well as palaeopathological analysis, for anthropological
material from archaeological excavations. Review of skeletal material
available at the Repository of Bioarchaeological Material, LULVI, was
also started.
- The collaboration partner, Latvian Biomedical
Research and Study
Centre (BMC) identified the strategy for the extraction and analysis of
ancient DNA (aDNA) for the project, and prepared mitochondrial DNA
(mtDNA) libraries of people involved in handling project samples, to be
used for checking contamination from most likely sources.
- During the public event, European Researchers’
Night 2018,
members of the public interested in the work of the BMC were given an
insight into the opportunities of aDNA analysis for archaeological
material.
- The project’s management committee was formed
from the LULVI and
BMC representatives, and the leaders of project activities.
Date information
prepared: 30.12.2018
January
1, 2019 – April 30, 2019
- Data review was continued on the middle and late
Latvian Iron Age
burial grounds (7th – 12th centuries AD), including
archaeological
reports, published data, etc., at the Repository of Archaeological
Material, Institute of Latvian History, University of Latvia (LULVI)
and the repository of archaeological material, Museum of National
History.
- Bioarchaeological analysis was started on the
available
anthropological material, as well as collection of samples for the
various research activities planned in the project.
- DNA was extracted from the first project samples;
modern mtDNA
libraries were sequenced; for the project samples, libraries were
prepared, and quality control was carried out. Modification and testing
of DNA extraction protocol for ancient samples, and modification and
testing of aDNA library preparation protocol to reduce loss of sample
DNA, was performed.
- Two abstracts were submitted for participation at the
25th Annual
Meeting of the EAA, Going Beyond Paradigms, session “Systemic
Approaches to Juvenile Funerary Rituals. Atypical, Deviant or
Normative?”.
- In cooperation with the National Heritage Board of
Latvia, expert
reports (A. Vilcāne) were provided about preservation of the Iron Age
archaeological material.
- For publicity purposes, a public event, The Shadow
Day was
organised at the BMC, where interested members of the public were
introduced to the possibilities and results of aDNA analysis.
- A review of scientific literature relevant for the
project activities, was carried out.
Date information
prepared: 30.04.2019
May 1,
2019 – August 31, 2019
- Research and analysis of archaeological information
gathered
during the data review about the Lejasbitēni and Čunkāni –
Dreņģeri
burial grounds (burial traditions, grave goods, etc.), was continued.
- Palaeopathological analysis, as well as age
determination of
non-adult individuals was started on available Iron Age anthropological
material. Collection of samples for the various analyses planned in the
project was continued.
- At the aDNA laboratory, BMC, work was continued on
aDNA
extraction, preparation of libraries, sex determination using PCR
methods, and modification and testing of aDNA library preparation
protocol to reduce loss of sample DNA.
- Collection of the first environmental samples (soil)
from Iron
Age burial grounds was started, in order to determine microbiome
profiles with the Ion Torrent Next Generation Sequencing technologies.
- An abstract was submitted for the participation at
the
international conference, „Investigating bones: Diet, health,
environment in the Baltic region” in Vilnius, Lithuania.
- The Viļaka Municipality Council was consulted with
regard to the
preservation of archaeological heritage from the local area, as well as
rescue excavations at the Logini ancient burial ground. During the
excavations, Iron Age samples were collected for the ancient microbiome
analysis.
- Review of scientific literature relevant to the
project activities was continued.
Date information
prepared: 31.08.2019
September
1, 2019 – December 31, 2019
- Collection and analysis of archaeological data was
continued.
Female gender and non-adult burials containing weapons were studied in
more detail.
- In cooperation with Poznan Radiocarbon Laboratory,
Poland, the
first six dates from two Iron Age burial grounds were obtained.
- The collection and processing of samples was
continued, and stable isotope analysis was started.
- The work on aDNA was continued, with activities
including whole
genome enrichment and library sequencing, sex determination using PCR
methods and sequencing the data, and testing of a new aDNA extraction
method from human teeth.
- Samples from Iron Age burial environments (soil,
dental calculus)
were analysed. Extraction of aDNA and shotgun sequencing was performed
on several human tooth samples.
- The results obtained during the project were
presented in the
following international conferences: 25th Annual Meeting of the EAA,
Going Beyond Paradigms, in Bern, Switzerland, September 4-7 (two
presentations); Investigating bones: Diet, health, environment in the
Baltic region, October 4-5, 2019, Vilnius, Lithuania; International
Scientific Conference, History and archaeology of the Daugava region,
November 19 – 22, in Minsk, Belarus.
- The following abstracts were published:
1) “Axe in the
Iron Age Child Burials (7th – 12th century AD) in the
territory of Latvia: Gender, Sex or Status” / Aija Ērkšķe,
Antonija
Vilcāne, Elīna Pētersone-Gordina, Alisa Kazarina, Jānis Ķimsis, Renāte
Ranka, Guntis Gerhards // 25th EAA Annual Meeting, 25 years Beyond
Paradigms, September 4-7 2019, Bern: Abstract Book, Bern: European
Association of Archaeologists, 2019, p. 64-65.
2) “Sex determination of Iron Age Human Remains from
Latvia” / Jānis
Ķimsis, Elīna Pētersone-Gordina, Alisa Kazarina, Antonija Vilcāne, Aija
Ērkšķe, Egija Zole, Guntis Gerhards, Renāte Ranka //
Investigating
bones: Diet, health, environment in the Baltic region: 10th
International Conference of Prof. Jonas Puzinas, October 4-5, 2019,
Vilnius: Conference Proceedings, Vilnius: Vilnius University, 2019, p.
23.
3) “Two deviant subadult burials at the Čunkāni-Dreņģeri
cemetery
(8th-11th c.) in the territory of Latvia” / Aija Ērkšķe //
25th EAA
Annual Meeting, 25 years Beyond Paradigms, September 4-7, 2019, Bern:
Abstract Book, Bern: European Association of Archaeologists, 2019, p.
64.
- Popular Science publications about the project:
1) Ērkšķe, A.
“Ieskats Eiropas arheologu asociācijas ikgadējā
konferencē Bernē, Šveicē” [An insight into the Annual
Meeting of the
EAA in Bern, Switzerland] // Zinātnes vēstnesis 15 (578), ISSN
1407-6748, September 30, 2019.
2) Vilcāne, A. “Starptautiska zinātniska konference par
piedaugavas
vēsturi un arheoloģiju Baltkrievijā” [An international scientific
conference about the history and archaeology of areas near the river
Daugava in Belarus] // Zinātnes vēstnesis 21 (584), ISSN 1407-6748,
December 23, 2019.
3) Keynote speech (J. Ķimsis), public lecture at the conference
organised by the students of the University of Latvia, Homo Et, titled
„Feedback: human and innovation”.
4) Work was continued on evaluation of data obtained during the
project, as well analysis of relevant scientific literature, and
preparation of manuscripts for publication.
Date information prepared: 30.12.2019
January
1, 2020 –February 29, 2020
- Project activities with regard to analysis of
archaeological
material, bioarchaeological analysis, and stable isotope and aDNA
analyses, were continued.
- The project results were presented at the
International
Scientific Conference, XXX Scientific Readings, at Daugavpils
University, January 26 – 27, Daugavpils, Latvia (two
presentations).
- The following manuscripts were submitted for
publication:
1) Ērkšķe, A.
“Children are missing! Some thoughts about the
underrepresentation of non–adult burials in Latvian Iron Age
cemeteries” (Journal of Estonian archaeology; Web of Science).
2) Gerhards, G. “Gender and status in Iron Age Latgalian
society”
(in Latvian; the peer-reviewed collection of articles Vēsture: avoti un
cilvēki [History: Sources and People].
3) Vilcāne, A. “Funerary traditions near the north-eastern
border of
Latvia in late Iron Age and early medieval period” (in Latvian;
submitted to the peer-reviewed collection of articles, Vēsture: avoti
un cilvēki [History: Sources and People].
4) Ērkšķe, A., Vilcāne, A., E. Pētersone – Gordina,
Gerhards, G.
“Trephination only for the privileged? Case studies from the Iron
Age
Latvia (7th-10th c AD)” (in Latvian; submitted to peer-reviewed
journal
Arheoloģija un Etnogrāfija [Archaeology and Ethnography]).
- In cooperation with the National Heritage Board of
Latvia expert
reports (A. Vilcāne) were provided about dating of the Iron Age
archaeological material.
- Collaboration was commenced with European
laboratories (Germany,
Estonia) with regard to aDNA analysis of control samples of the project
material.
- An abstract was submitted for the participation at
the 26th
Annual Meeting of the EAA, session "More than Just Bones -
Understanding Past Human Behaviour through the Study of Human
Remains”,
in Budapest, Hungary.
Date information
prepared: 29.02.2020
March 1, 2020 May 31, 2020 1.
Due to COVID-19 state of emergency was announced in Latvia and
elsewhere in Europe. The epidemiological safety measures
significantly affected data collection in museums and repositories,
as well as the functioning of laboratories. Planned conferences were
either cancelled or postponed. 2. Archaeological material from
the Lejasbitēni cemetery (453 burials) was systematically grouped in
order to enable statistical analysis of burial traditions. 3.
Palaeopathological analyses, as well as age estimation were
continued on available Iron Age anthropological material. 4. The
work for aDNA extraction, purification and sequencing was performed
as planned. In most cases the archaeological samples from
Lejasbitēni burials were chosen for the aDNA analysis. 5. Some
adjustments for the bioinformatics pipelines have been made in
accordance with the most recent aDNA protocols available in
scientific literature. The analysis of aDNA data was performed, and
obtained results were compared for different protocols/sample
groups. 6. Review of scientific literature relevant to the
project activities was continued.
Date information
prepared: 31.05.2020
June 1, 2020
August 31, 2020 1. In response to the continuation of
COVID-19 pandemic as a force majeure situation, the project
management committee amended the schedule of project activities, as
well as the budget, whereby the funds which had been allocated for
travel (conferences, and capacity building for the scientific team
in laboratories abroad) were reallocated for additional radiocarbon
analysis, as well as amelogenin peptide analysis for sex
determination in archaeological human tooth samples. 2.
Archaeological material from the Čunkāni Dreņģeri cemetery (743
burials) was systematically grouped, in order to enable statistical
analysis of burial traditions. 3. Analysis of bioarchaeological
data from Iron Age (7th 11th AD) cemeteries was continued. 4.
The work for aDNA extraction, purification and sequencing was
performed as planned. In most cases the archaeological samples from
Čunkāni Dreņģeri burials were chosen for the aDNA analysis. 5.
The bioinformatics analysis pipelines were amended for
microbiome/ancient pathogen studies based on aDNA data, and
microbiome analysis of archaeological samples was performed. 6.
The results obtained during the project were presented in the
following international conference: 26th EAA Virtual Annual Meeting,
August 24-30. 7. The following abstract was published: Ērkšķe,
A., Vilcāne, A., Pētersone-Gordina, E. Gerhards, G. Trephination
only for the privileged? Case studies from the Iron Age Latvia (7th
10th CC AD) / // 26th EAA Virtual Annual Meeting, August 24-30,
2020: Abstract Book Prague: European Association of Archaeologists,
2020. P.565-565; ISBN 9788090727076. 8. A paper about non-adult
individuals in Iron Age cemeteries from Latvia was published:
Ērkšķe, A. 2020. The children are missing! Some thoughts on the
underrepresentation of non-adult burials in Latvian Iron Age
cemeteries. Estonian Journal of Archaeology. Vol 24, N 2,
pp. 161-189.
doi.org/10.3176/arch.2020.2.03.
Date
information prepared: 31.08.2020
September 1, 2020 December 31, 2020
1. Work was continued in the repository of archaeological material
at the National History Museum of Latvia to obtain comparative data
about Iron Age Latgalian and Semigallian cemeteries. 2. Tooth
samples were collected for amelogenin peptide analysis for sex
determination in archaeological human remains. 3. In cooperation
with the Stable Isotope Biogeochemistry Laboratory (SIBL),
Department of Archaeology, Durham University, Great Britain, the
first stable isotope results from Latvian Iron Age cemeteries were
obtained. 4. Standard aDNA analysis workflow was slightly
amended for aDNA extraction purposes from poorly preserved
archaeological samples. 5. Additional archaeological samples
from previously studied individuals were obtained for those cases
when the initial aDNA samples were of low quality/showed excessive
degradation pattern. 6. The analysis of aDNA sequencing data was
performed; both human and microbial aDNA data were assessed. 7.
The general public was informed about the implementation of this
research project, and the importance of aDNA research field was
highlighted during the interview for a local radio channel.
https://lr2.lsm.lv/lv/raksts/nakotnes-pietura/renates-rankas-interesu-loka-seno-infekcijas-slimibu-izpete.a137422/
[from 14:10]
Date information prepared:
31.12.2020
January 1, 2021 April
2021 1. Analysis of human remains from the
Lejasbitēni and Čunkāni Drenģeri cemeteries, as well as the study
of burial traditions with regard to gender, sex, and social status,
was continued. The typological dating of grave goods, and the
estimation of individual social status, was carried out. 2.
Overall, aDNA isolation, sequencing and data analysis was
continuing. 3. For separate archaeological sample sets
biological sex of the individual was assessed based on aDNA data.
4. Metagenomics datasets were thoroughly analysed for the
archaeological tooth samples, traces of ancient oral microbiome were
studied, and authentication of oral microbial aDNA was performed.
5. The goals, methodology, challenges and benefits of aDNA
research field were described and explained at the public event
during the 2021 European Researchers' Night (https://www.facebook.com/LatvianBiomedicalCentre/videos/eiropas-zin%C4%81tnieku-nakts-2021-lbmc/368266991185218/
[from 43:00]
https://www.youtube.com/watch?v=HsdYBlqu8Ps) The advancement of
aDNA research in Latvia and the contribution of this project was
highlighted. 6. The project results were presented at
International Scientific Conferences, XXXI Scientific Readings, at
Daugavpils University, January 28 29, Daugavpils, Latvia (two
presentations); 79th International Scientific Conference of the
University of Latvia on Medicine, April 24, Riga, Latvia. 7. The
following abstract was published: Ķimsis, J., Kazarina, A.,
Gerhards, G., Ranka, R. 2021. Metagenome analysis of archaeological
teeth samples from Latvian Iron Age, 8th -11th centuries AD (Poster
Presentations. Basic Medical Science) 79th International Scientific
Conference of the University of Latvia on Medicine April 23, p. 49.
8. The following manuscripts were submitted for publication:
Ķimsis, J. Ranka, R., Vilcāne, A. Gehards, G. Molecular sex
determination in an ambiguous burial from Latvian Iron Age (in
Latvian, the peer-reviewed collection of articles Vēsture: avoti un
cilvēki [History: Sources and People]. Ērkšķe, A. Bracelets made
from other jewellery as a source about subadults social status in
the Iron Age society: case study of the Čunkāni-Dreņģeri cemetery
(8th 11th cc AD) (in Latvian, the peer-reviewed collection of
articles Vēsture: avoti un cilvēki [History: Sources and People].
Date information prepared: 31.04.2021
May 1, 2021 August 31, 2021
1. In
cooperation with Poznan Radiocarbon Laboratory, Poland, new dates
from Lejasbitēni and Čunkāni Drenģeri cemeteries were obtained.
2. In cooperation with the Stable Isotope Biogeochemistry
Laboratory (SIBL), Department of Archaeology, Durham University,
Great Britain, new stable isotope results, and the first results of
the amelogenin peptide analysis from Latvian Iron Age cemeteries
were obtained. 3. Work was started on evaluation of all
material from the Lejasbitēni and Čunkāni Drenģeri cemeteries in
the light of data obtained from dietary stable isotope and aDNA
analyses. 4. Isolation and purification of aDNA from the final
archaeological sample sets was performed, the quality of aDNA
samples was assessed. 5. Sequencing libraries were prepared,
additional size selected, and library enrichment was performed, if
necessary. 6. Sequencing of aDNA libraries using Illumina
methodology was performed, and raw sequencing data were processed
using various bioinformatics tools/pipelines. 7. Work was
continued on evaluation of data obtained during the project, as well
analysis of relevant scientific literature, and preparation of
manuscripts for publication. 8. The project results were
presented at the scientific readings of the National History Museum
on 14th May: Vilcāne A., Orientācijas nozīme apbedīšanas tradīcijās.
Ieskats historiogrāfijā [The significance of burial orientation in
funerary ritual in historiography].
Date information
prepared: 31.08.2021
September 1,
2021 December 31, 2021 1. Work was continued
on studying material from the Lejasbitēni and Čunkāni Drenģeri
cemeteries in the light of data obtained from dietary stable
isotope, and bioarchaeological analyses, in order to assess the
quality of life of these populations. All results were processed and
analysed, and relevant manuscripts were prepared. 2. The
obtained aDNA sequencing data sets were thoroughly assessed by using
several bioinformatics tools, pipelines, and analysis methods.
3. Human aDNA data sets were rigorously analysed. The authentication
of human aDNA was performed, human aDNA amount/degradation in
various samples was assessed. For individuals, biological sex
identification and mitochondrial DNA analysis was performed. 4.
Microbial metagenome datasets were analysed, bacterial diversity and
characteristic features in various archaeological samples from two
burial sites were evaluated. The possible presence of ancient
pathogens was assessed. 5. The obtained results were presented
at the conference: 27th EAA Annual Meeting, Biosocial Archaeology:
When Ancient DNA Opens the Discussion to Social Structures.
Assembling archaeological theory and the archaeological sciences
(Widening Horizons, Virtual Meeting), September 6-11, 2021, Kiel. An
abstract was published: Ranka, R., Ķimsis, J., Pokšāne, A.,
Pētersone-Gordina, E., Vilcāne, A., Kazarina, Gerhards, G. 2021
Survival of aDNA in Tooth and Bone Samples from Iron Age (7th -12th
Centuries AD) Burials in Latvia. //27th EAA Annual Meeting (Abstract
Book Prague: European Association of Archaeologists, P.716. 6.
Scientific manuscripts (4) were prepared and submitted to journals
which are included in Scopus/ Web of Sciences databases.
Date information prepared: 30.12.2021 |